High Prevalence of Antiseptic Resistance Encoding Genes and Reduced Phenotypic Antiseptic Susceptibility Among Antibiotic-Resistant Pseudomonas aeruginosa Isolates

Background: Multi-drug resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa isolates are of clinical concern. Objectives: To determine the distribution antiseptic resistance genes associated level phenotypic against quaternary ammonium compounds biguanide compounds, we studied MDR XDR P. collected from different infections among patients a single hospital. Methods: were investigated in 2020 for vitro susceptibility to benzethonium chloride (BTC), benzalkonium (BKC), chlorhexidine digluconate (CHG). The minimum inhibitory concentrations (MICs) these agents determined using broth microdilution. Also, polymerase chain reaction (PCR)-mediated detection qacE, qacEΔ1, blaOXA-23 was used. Results: Isolates largely non-clonal according their phenotypical genotypical non-similarity (35 overall data-combination types detected). Most occurred intensive care unit (ICU) (n = 43, 61.4%). Extensively phenotypes detected 20% 12.6%, respectively. Among 70 retained, 53 (75.7%) harbored at least one gene, comprising 11 (20.7%) with solely qacEΔ1 gene; seven (13.2%) qacE gene. Both simultaneously 35 (66%) isolates. mean MICs BTC (24.0 versus 10.56 µg/mL), BKC (46.1 17.22 CHG (107.7 29.4 µg/mL) statistically significantly higher gene harboring than other without such genes. Conclusions: increased antibiotic-resistant highlight importance monitoring increases implementing effective infection control.